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Sample GSM202681 Query DataSets for GSM202681
Status Public on Jun 21, 2007
Title Mammary Gland @ Preg12, biological rep4
Sample type RNA
 
Source name Mammary Gland @ Preg12, biological rep4
Organism Mus musculus
Characteristics Genotype:FVB
Treatment protocol no treatments
Growth protocol mice were grown at the University of Colorado vivarium on a standard laboratory chow diet
Extracted molecule total RNA
Extraction protocol the fourth mammary glands on mice were excised and the lymph nodes were removed, samples were stored in RNA Later from Qiagen according to manufacturers instructions. Total RNA was isolated using RNA easy from qiagen according to their protocol, and total RNA was examined for purity using a nanodrop and for integrity using agilent bioanalyzer.
Label biotin
Label protocol all samples were biotin labeled using the ENZO labeling kit and protocol according to affymetrix parameters beginning from 2ug of total RNA converted to cDNA
 
Hybridization protocol Hybridization protocol according to affymetrix. Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mu74Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scan protocol according to affymetrix on GeneChip Scanner 3000
Description Gene expression data from mammary glands of pregnant mice
Data processing Samples were evaluated for quality control parameters using Gene Chip Operating System from Affymetrix. CEL files were then imported into GeneSpring (Agilent Technologies) and processed using their native GC-RMA algorithm.
 
Submission date Jun 20, 2007
Last update date Jun 06, 2022
Contact name Michael C. Rudolph
E-mail(s) Michael-rudolph@ouhsc.edu
Organization name OUHSC
Department Physiology
Lab Rudolph Lab
Street address 975 NE 10th St. BRC North rm 364
City Oklahoma City
State/province OK
ZIP/Postal code 73104
Country USA
 
Platform ID GPL81
Series (1)
GSE8191 Key stages in mammary gland development.
Relations
Reanalyzed by GSE31291

Data table header descriptions
ID_REF
VALUE GC-RMA normalized
StdErr Norm

Data table
ID_REF VALUE StdErr Norm
100001_at 0.924137 0.17378676
100002_at 0.43286762 0.17385298
100003_at 0.49592954 0.173853
100004_at 1.2353764 0.1737888
100005_at 1.9421539 0.1737797
100006_at 0.8004263 0.17382644
100007_at 1.6823744 0.1737802
100009_r_at 0.6849675 0.17385298
100010_at 0.89922535 0.17377968
100011_at 0.9143101 0.17377947
100012_at 1.3279592 0.17377955
100013_at 1.3221378 0.17377934
100014_at 0.9611335 0.17378005
100015_at 0.9162088 0.17378011
100016_at 0.8984427 0.17382924
100017_at 0.7259484 0.173853
100018_at 0.8479118 0.1737834
100019_at 1.8518702 0.17379868
100020_at 1.0070055 0.17377996
100021_at 0.84644735 0.17383593

Total number of rows: 12488

Table truncated, full table size 376 Kbytes.




Supplementary file Size Download File type/resource
GSM202681.CEL.gz 2.8 Mb (ftp)(http) CEL
GSM202681.CHP.gz 4.8 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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