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Sample GSM202690 Query DataSets for GSM202690
Status Public on Jun 21, 2007
Title Mammary Gland @ Lac1, biological rep1
Sample type RNA
Source name Mammary Gland @ Lac1, biological rep1
Organism Mus musculus
Characteristics Genotype:FVB
Treatment protocol no treatments
Growth protocol mice were grown at the University of Colorado vivarium on a standard laboratory chow diet
Extracted molecule total RNA
Extraction protocol the fourth mammary glands on mice were excised and the lymph nodes were removed, samples were stored in RNA Later from Qiagen according to manufacturers instructions. Total RNA was isolated using RNA easy from qiagen according to their protocol, and total RNA was examined for purity using a nanodrop and for integrity using agilent bioanalyzer.
Label biotin
Label protocol all samples were biotin labeled using the ENZO labeling kit and protocol according to affymetrix parameters beginning from 2ug of total RNA converted to cDNA
Hybridization protocol Hybridization protocol according to affymetrix. Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mu74Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scan protocol according to affymetrix on GeneChip Scanner 3000
Description Gene expression data from mammary glands of lactating mice
Data processing Samples were evaluated for quality control parameters using Gene Chip Operating System from Affymetrix. CEL files were then imported into GeneSpring (Agilent Technologies) and processed using their native GC-RMA algorithm.
Submission date Jun 20, 2007
Last update date Jun 06, 2022
Contact name Michael C. Rudolph
Organization name OUHSC
Department Physiology
Lab Rudolph Lab
Street address 975 NE 10th St. BRC North rm 364
City Oklahoma City
State/province OK
ZIP/Postal code 73104
Country USA
Platform ID GPL81
Series (1)
GSE8191 Key stages in mammary gland development.
Reanalyzed by GSE31291

Data table header descriptions
VALUE GC-RMA normalized
StdErr Norm

Data table
100001_at 1.2128491 0.15430194
100002_at 0.6011759 0.1543705
100003_at 0.66651195 0.15437052
100004_at 1.490923 0.15430406
100005_at 1.4251682 0.15429461
100006_at 1.2243888 0.15434304
100007_at 0.7925033 0.15429515
100009_r_at 1.117374 0.1543705
100010_at 0.7378663 0.15429462
100011_at 0.8950444 0.1542944
100012_at 0.4552483 0.15429446
100013_at 1.3654112 0.15429424
100014_at 0.84717786 0.154295
100015_at 0.9277667 0.15429506
100016_at 1.033919 0.15434593
100017_at 0.9600064 0.15437052
100018_at 1.139748 0.15429845
100019_at 0.88188404 0.15431428
100020_at 1.127582 0.15429491
100021_at 1.2968371 0.15435284

Total number of rows: 12488

Table truncated, full table size 377 Kbytes.

Supplementary file Size Download File type/resource
GSM202690.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM202690.CHP.gz 4.2 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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