|
| Status |
Public on Oct 11, 2016 |
| Title |
evolved 5/ ancestral 5 cells, replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
S. cerevisiae polyploid cells at 1000 generations
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain/background: W303
ploidy: polyploid
sample type: evolved
|
| Growth protocol |
Cells were grown to log phase in YPD medium at 36 degrees Celsius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy Midi Kit (Qiagen, Valencia, CA).
|
| Label |
Alexa Fluor 647
|
| Label protocol |
The labeling protocol was adapted from Microarray Core Facility, Institute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html).
|
| |
|
| Channel 2 |
| Source name |
S. cerevisiae polyploid cells, ancestral
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain/background: W303
ploidy: polyploid
sample type: ancestral
|
| Growth protocol |
Cells were grown to log phase in YPD medium at 36 degrees Celsius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy Midi Kit (Qiagen, Valencia, CA).
|
| Label |
Alexa Fluor 555
|
| Label protocol |
The labeling protocol was adapted from Microarray Core Facility, Institute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html).
|
| |
|
| |
| Hybridization protocol |
The hybridization protocol was adapted from Microarray Core Facility, Institute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html).
|
| Scan protocol |
Arrays were scanned for F635 (Alexa Fluor 647) and F532 (Alexa Fluor 555) fluorescence intensities using AXON 4000B (Molecular Device, Sunnyvale, CA).
|
| Description |
Sample name: evo5-rep1
Technical replicate 1 of 3. Evolved/ancestral cells.
|
| Data processing |
LOWESS normalized, background subtracted data obtained from signals. Agilent GeneSpring GX 7.3 was used.
|
| |
|
| Submission date |
Jan 06, 2016 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Jun-Yi Leu |
| E-mail(s) |
jyl9216public@gmail.com
|
| Organization name |
Institute of Molecular Biology
|
| Lab |
N411
|
| Street address |
No. 128, Sec. 2, Academia Rd., Nangang Dist.
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL18498 |
| Series (2) |
| GSE76596 |
Whole genome gene expression analysis of evolved polyploid Saccharomyces cerevisiae cell lines at 36 degrees Celsius |
| GSE76597 |
Karyotype analysis and whole genome gene expression analysis of evolved polyploid Saccharomyces cerevisiae cell lines |
|
