|
Status |
Public on Jun 22, 2007 |
Title |
R439P00089 |
Sample type |
RNA |
|
|
Source name |
prostate cancer sample
|
Organism |
Homo sapiens |
Characteristics |
Percentage of Tumor: 0% Percentage of Stroma: 94% Percentage of BPH: 4% Percentage of Atrophic Gland: 2%
|
Extracted molecule |
total RNA |
Extraction protocol |
QiaGen Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Scanner.
|
Description |
prostate
|
Data processing |
The data were analyzed and normalized with RMA.
|
|
|
Submission date |
Jun 21, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Yipeng Wang |
E-mail(s) |
ywang@sdibr.org
|
Phone |
8585813960
|
Organization name |
Vaccine Research Institute of San Diego
|
Street address |
|
City |
San Diego |
State/province |
CA |
ZIP/Postal code |
92121 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE8218 |
Gene expression data from prostate cancer samples |
|