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Sample GSM2033392 Query DataSets for GSM2033392
Status Public on May 31, 2016
Title TMK2
Sample type RNA
 
Source name Tympanic membrane keratinocyte
Organism Homo sapiens
Characteristics tissue: tympanic membrane
cell type: keratinocyte
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
Label biotin
Label protocol Total RNA (300 ng) from each sample was prepared using the Genisphere FlashTag HSR kit(Genisphere, Hatfield, PA) according to manufacturer’s instructions.
 
Hybridization protocol Labeled sense strand DNA was hybridized to the GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
Scan protocol Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
Description Primary cells from surgical patients
Data processing The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization.
miRNA-3_0-st-v3.pgf​
miRNA-3_0.annotations.psname.20121203.probeset.csv
 
Submission date Jan 08, 2016
Last update date May 31, 2016
Contact name Peder Aabel
E-mail(s) peder.aabel@medisin.uio.no
Organization name Akershus University Hospital
Department ENT
Street address Sykehusvn. 27
City Lorenskog
ZIP/Postal code 1478
Country Norway
 
Platform ID GPL16384
Series (2)
GSE76673 Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]
GSE76674 Expression data of human tympanic membrane cells and epidermal keratinocytes

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
14q0_st 0.911948
14qI-1_st 1.05721
14qI-1_x_st 0.959444
14qI-2_st 1.21937
14qI-3_x_st 0.79529
14qI-4_st 0.964691
14qI-4_x_st 1.08492
14qI-5_st 0.903467
14qI-6_st 1.15152
14qI-6_x_st 0.842298
14qI-7_st 1.19022
14qI-8_st 0.910749
14qI-8_x_st 1.12856
14qI-9_x_st 1.04985
14qII-10_st 1.02583
14qII-10_x_st 1.19022
14qII-11_st 0.900885
14qII-11_x_st 1.03053
14qII-12_st 0.885891
14qII-12_x_st 1.19382

Total number of rows: 25119

Table truncated, full table size 623 Kbytes.




Supplementary file Size Download File type/resource
GSM2033392__52090200931520121714418819660422.CEL.gz 806.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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