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Sample GSM2033394 Query DataSets for GSM2033394
Status Public on May 31, 2016
Title EK1
Sample type RNA
 
Source name Epidermal keratinocyte
Organism Homo sapiens
Characteristics tissue: epidermis
cell type: keratinocyte
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
Label biotin
Label protocol Total RNA (300 ng) from each sample was prepared using the Genisphere FlashTag HSR kit(Genisphere, Hatfield, PA) according to manufacturer’s instructions.
 
Hybridization protocol Labeled sense strand DNA was hybridized to the GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
Scan protocol Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
Description Purchased cells from ScienCell, HEK-a
Data processing The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization.
miRNA-3_0-st-v3.pgf​
miRNA-3_0.annotations.psname.20121203.probeset.csv
 
Submission date Jan 08, 2016
Last update date May 31, 2016
Contact name Peder Aabel
E-mail(s) peder.aabel@medisin.uio.no
Organization name Akershus University Hospital
Department ENT
Street address Sykehusvn. 27
City Lorenskog
ZIP/Postal code 1478
Country Norway
 
Platform ID GPL16384
Series (2)
GSE76673 Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]
GSE76674 Expression data of human tympanic membrane cells and epidermal keratinocytes

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
14q0_st 0.910564
14qI-1_st 0.881225
14qI-1_x_st 1.17074
14qI-2_st 1.24957
14qI-3_x_st 1.12946
14qI-4_st 0.809156
14qI-4_x_st 0.962341
14qI-5_st 1.02033
14qI-6_st 1.00454
14qI-6_x_st 0.889808
14qI-7_st 1.41107
14qI-8_st 0.80078
14qI-8_x_st 0.906587
14qI-9_x_st 0.889457
14qII-10_st 0.929528
14qII-10_x_st 1.02156
14qII-11_st 0.974664
14qII-11_x_st 1.1525
14qII-12_st 0.8318
14qII-12_x_st 0.766835

Total number of rows: 25119

Table truncated, full table size 622 Kbytes.




Supplementary file Size Download File type/resource
GSM2033394__52090200931520121714418819660443.CEL.gz 795.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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