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Sample GSM2033395 Query DataSets for GSM2033395
Status Public on May 31, 2016
Title EK2
Sample type RNA
Source name Epidermal keratinocyte
Organism Homo sapiens
Characteristics tissue: epidermis
cell type: keratinocyte
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
Label biotin
Label protocol Total RNA (300 ng) from each sample was prepared using the Genisphere FlashTag HSR kit(Genisphere, Hatfield, PA) according to manufacturer’s instructions.
Hybridization protocol Labeled sense strand DNA was hybridized to the GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
Scan protocol Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
Description Purchased cells from ScienCell, HEK-a
Data processing The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization.
Submission date Jan 08, 2016
Last update date May 31, 2016
Contact name Peder Aabel
Organization name Akershus University Hospital
Department ENT
Street address Sykehusvn. 27
City Lorenskog
ZIP/Postal code 1478
Country Norway
Platform ID GPL16384
Series (2)
GSE76673 Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]
GSE76674 Expression data of human tympanic membrane cells and epidermal keratinocytes

Data table header descriptions
VALUE RMA signal

Data table
14q0_st 0.971694
14qI-1_st 0.940248
14qI-1_x_st 1.3144
14qI-2_st 1.38142
14qI-3_x_st 1.07974
14qI-4_st 1.24664
14qI-4_x_st 1.02351
14qI-5_st 0.903467
14qI-6_st 1.06709
14qI-6_x_st 0.957242
14qI-7_st 1.07432
14qI-8_st 1.13965
14qI-8_x_st 0.840327
14qI-9_x_st 1.19385
14qII-10_st 1.37777
14qII-10_x_st 1.17266
14qII-11_st 1.4868
14qII-11_x_st 1.22456
14qII-12_st 1.13158
14qII-12_x_st 0.875229

Total number of rows: 25119

Table truncated, full table size 623 Kbytes.

Supplementary file Size Download File type/resource
GSM2033395__52090200931520121714418819660430.CEL.gz 796.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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