GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2033396 Query DataSets for GSM2033396
Status Public on May 31, 2016
Title EK3
Sample type RNA
Source name Epidermal keratinocyte
Organism Homo sapiens
Characteristics tissue: epidermis
cell type: keratinocyte
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Total RNA from samples of cultivated TMK and EK cells was extracted using the miRNeasy mini kit from Qiagen
Label biotin
Label protocol Total RNA (300 ng) from each sample was prepared using the Genisphere FlashTag HSR kit(Genisphere, Hatfield, PA) according to manufacturer’s instructions.
Hybridization protocol Labeled sense strand DNA was hybridized to the GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). The arrays were washed and stained using FS-450 fluidics station (Affymetrix).
Scan protocol Fluorescent images were scanned by Hewlett Packard Gene Array Scanner 3000 7G (Hewlett Packard, Palo Alto, CA).The scanned images were then analyzed using Affymetrix GeneChip Command Console
Description Purchased cells from ScienCell, HEK-a
Data processing The CEL files were imported into Partek Genomics Suite software (Partek, Inc., St. Louis, MO). Robust microarray analysis (RMA) was applied for normalization.
Submission date Jan 08, 2016
Last update date May 31, 2016
Contact name Peder Aabel
Organization name Akershus University Hospital
Department ENT
Street address Sykehusvn. 27
City Lorenskog
ZIP/Postal code 1478
Country Norway
Platform ID GPL16384
Series (2)
GSE76673 Expression data of human tympanic membrane cells and epidermal keratinocytes [miRNA]
GSE76674 Expression data of human tympanic membrane cells and epidermal keratinocytes

Data table header descriptions
VALUE RMA signal

Data table
14q0_st 1.03664
14qI-1_st 1.04328
14qI-1_x_st 0.902665
14qI-2_st 1.48372
14qI-3_x_st 1.23552
14qI-4_st 0.869038
14qI-4_x_st 1.17569
14qI-5_st 1.06757
14qI-6_st 1.73782
14qI-6_x_st 1.21996
14qI-7_st 1.42811
14qI-8_st 0.946145
14qI-8_x_st 1.06533
14qI-9_x_st 1.08262
14qII-10_st 1.08516
14qII-10_x_st 1.50992
14qII-11_st 1.39793
14qII-11_x_st 0.938599
14qII-12_st 0.985919
14qII-12_x_st 0.977756

Total number of rows: 25119

Table truncated, full table size 621 Kbytes.

Supplementary file Size Download File type/resource
GSM2033396__52090200931520121714418819660456.CEL.gz 768.8 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap