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| Status |
Public on Jun 22, 2017 |
| Title |
Exp100_d7_EPCR+_1 |
| Sample type |
SRA |
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| Source name |
Exp100_d7_EPCR+
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| Organism |
Homo sapiens |
| Characteristics |
cell type: CD34+CD45RA- cord blood cells
protocol: 7 day expansion in UM171
sorted cell population: EPCR+
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| Growth protocol |
CD34+CD45RA- cord blood derived cells were cultured for 7 days in serum free media with cytokines (100ng/ml SCF, 100ng/mlFLT3, 50ng/ml TPO) supplemented with UM171 (38nM)
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA extraction using trizol was performed on the sorted population: EPCR+, EPCRlow, EPCR- using RNeasy mini Kit (Qiagen, #74106)
TruSeq RNA Sample Preparation (Illumina)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Basecalls performed using CASAVA 1.8.2
Sequenced reads were masked for sequencing adapters and mapped to the reference genome using CASAVA 1.8.2
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CASAVA 1.8.2
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample. Columns are: Chromosome, Start location, Stop location, Gene, RPKM, base count.
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| Submission date |
Jan 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Guy Sauvageau |
| Organization name |
Institute for Research in Immunology and Cancer
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| Street address |
2950, Chemin de Polytechnique
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| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3T 1J4 |
| Country |
Canada |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE77128 |
EPCR Expression Defines the Most Primitive Subset of Human HSPC and Is Required for Their In Vivo Activity |
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| Relations |
| BioSample |
SAMN04435332 |
| SRA |
SRX1541748 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2044607_Exp100_d7_EPCR+_1_RPKM.txt.gz |
471.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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