|
| Status |
Public on Oct 02, 2017 |
| Title |
HF1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
LM-PCR products from HpaII digested DNA
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild type
tissue: Liver
strain: CD1
fraction: HpaII digested DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
High molecular weight genomic DNA was extracted from liver in mouse exposure to a control or high fat (HF) diet in utero
|
| Label |
Cy5
|
| Label protocol |
The samples were labeled for microarray analysis as described previously [Selzer, Genes Chromosomes Cancer 44:305–319] using Cy3 or Cy5-conjugated oligonucleotides and random primers.
|
| |
|
| Channel 2 |
| Source name |
LM-PCR products from MspI digested DNA
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild type
tissue: Liver
strain: CD1
fraction: MspI digested DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
High molecular weight genomic DNA was extracted from liver in mouse exposure to a control or high fat (HF) diet in utero
|
| Label |
Cy3
|
| Label protocol |
The samples were labeled for microarray analysis as described previously [Selzer, Genes Chromosomes Cancer 44:305–319] using Cy3 or Cy5-conjugated oligonucleotides and random primers.
|
| |
|
| |
| Hybridization protocol |
The HpaII and MspI representations were co-hybridized to the microarray and scanned to quantify the 532 and 635 nm fluorescence at each oligonucleotide on the microarray.
|
| Scan protocol |
see manufacturer's web site www.nimblegen.com
|
| Description |
DNA was extracted from the mouse exposure to a high fat diet in utero.
|
| Data processing |
Data analysis was performed using our published pipeline (Thompson, Reimers, Khulan, Gissot, Richmond, Chen, Zheng, Kim and Greally 2008), an open source resource available through BioConductor (HELP package)
|
| |
|
| Submission date |
Feb 01, 2016 |
| Last update date |
Oct 02, 2017 |
| Contact name |
Maureen J Charron |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Biochemistry
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL21388 |
| Series (2) |
| GSE77430 |
Alterations in the hepatic epigenome in mice exposed to a maternal high fat diet in utero [HELP] |
| GSE77432 |
Alterations in the hepatic epigenome in mice exposed to a maternal high fat diet in utero |
|
