|
| Status |
Public on Aug 01, 2016 |
| Title |
PAO1 Untreated biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PAO1 Untreated
|
| Organism |
Pseudomonas aeruginosa PAO1 |
| Characteristics |
agent: none
|
| Treatment protocol |
P. aeruginosa strain PAO1 were exposed to 0.3% (w/v) complex bile (Sigma).
|
| Growth protocol |
Three independent cultures of P. aeruginosa strain PAO1 were grown shaking from OD600nm 0.025 to 0.8 at 37 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Subsequently 500 μl of culture was treated with 1 ml of RNAProtect Bacteria Reagent (Qiagen). Total RNA was extracted using the RNAeasy kit (Qiagen) according to the manufacturer's instructions. To ensure DNA free RNA, the total RNA was DNase treated using TURBO DNase (Ambion).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 10 ug total RNA.
|
| |
|
| Hybridization protocol |
The labelled cDNA was hybridization to the Pseudomonas aeruginosa Affymetrix GeneChip Genome Array, which was subsequently washed and scanned.
|
| Scan protocol |
GeneChips were then scanned.
|
| Description |
Gene expression data from P. aeruginosa grown not in the presence of bile.
|
| Data processing |
The data were analyzed with Affymetrix' GeneChip Operating Software (GCOS).
|
| |
|
| Submission date |
Feb 08, 2016 |
| Last update date |
Aug 01, 2016 |
| Contact name |
Fergal O'Gara |
| E-mail(s) |
f.ogara@ucc.ie
|
| Phone |
353214902934
|
| Organization name |
BIOMERIT Research Centre
|
| Department |
Microbiology Dept, Univerisity College Cork
|
| Lab |
Lab 442
|
| Street address |
UCC, Western Rd
|
| City |
Cork |
| ZIP/Postal code |
0000 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE77665 |
Bile's chronic effect on the Cystic Fibrosis lung pathogen Pseudomonas aeruginosa |
|
