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Status |
Public on Dec 30, 2007 |
Title |
Chicken liver 6th week Sample 1 |
Sample type |
RNA |
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|
Source name |
chicken Liver 6 weeks of age
|
Organism |
Gallus gallus |
Characteristics |
Breed: Beijing-You chicken Gender: male Age: 6 week
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Biomaterial provider |
China Agricultural University
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissue samples were ground with pestle in a mortar under liquid nitrogen. Total RNA was extracted by using Trizol reagent from tissue powder, purified by using QIAquick PCR Purification kit.
|
Label |
Cy5
|
Label protocol |
For fluorescence labeling, 0.5 μl 1 M NaHCO3 and 1 μl Cy3 and Cy5 mono-reactive dyes (Amersham Pharmacia) in DMSO (Sigma) (10 μg/μl) were added to samples, respectively. The reaction mixture was then incubated at 37°C for 2 hours in the dark then purified by QIAquick PCR Purification kit to remove unincorporated fluorophores.
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|
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Hybridization protocol |
Two pmoles each of Cy3-labelled or Cy5-labelled sample in hybridization buffer was added to per isolated sub-array. cover the droplet with tiny cover slips with forceps, place the slide in a hybridization cassette and incubate the cassette for 16h at 42°C. washed in 2X SSC and 0.1% SDS (5 min, 55°C), 1X SSC and 0.1% SDS (5 min, 55°C), 0.1 X SSC (30sec, 3 X), and dried by centrifugation.
|
Scan protocol |
Fluorescent array images were collected individually in each sub-array area for Cy3 or Cy5 in a GenePix 4000B fluorescent scanner (Axon Instruments, CA)
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Description |
Indigenous chicken liver samples hybridized with samll group of genes which related to lipid metabolism.
|
Data processing |
Based onhousekeeping genes that are relatively invariant for printed cDNA microarry as positive control, an edited normalization method using Fortran software was used for for all data processing
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Submission date |
Jun 28, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Huifeng Li |
E-mail(s) |
lihuifengtom@yahoo.com.cn
|
Phone |
1-540-231-3183
|
Fax |
1-540-231-3010
|
Organization name |
Virginia Tech
|
Department |
Animal and Poultry Sciences
|
Lab |
Biotechnology
|
Street address |
3570 Litton Reaves Hall
|
City |
Blacksburg |
State/province |
VA |
ZIP/Postal code |
24061-0306 |
Country |
USA |
|
|
Platform ID |
GPL5442 |
Series (1) |
GSE8405 |
A new multi-sample hybridized microarray platform for quantitative gene expression on a small scale |
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