Strain: C57BL6 Pancreas, Female (MoPn.Feml) total RNA received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Extracted molecule
total RNA
Extraction protocol
RNA isolation Illumina mRNA QC passed
Description
Cells/tissue: Library lib_name Cell type Pancreas, Female (MoPn.Feml) Source NHGRI cDNA library: Library DpnII restriction - (signature captured by MmeI) Sequence length 20 bp CSMA Run Lane Clusters Total clusters successfully sequenced - 3309760 Processed Signatures - xxxxxxx
