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Sample GSM2070601 Query DataSets for GSM2070601
Status Public on Jun 12, 2017
Title Lsd1_ChIPSeq
Sample type SRA
Source name Trophoblast stem cells
Organism Mus musculus
Characteristics cell type: trophoblast stem cells
developmental stage: E3.5
passage: 15-20
antibody: 20752
Growth protocol TSCs medium suplemented with Fgf4 and Heparin
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
NEBNextUltra library preparation kit without size selection
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
Data processing ChIP-seq reads were aligned to the mm10 genome assembly using Bowtie.
peaks were called using MACS
Genome_build: mm10
Supplementary_files_format_and_content: wig files were generated using MACS
Submission date Feb 24, 2016
Last update date May 15, 2019
Contact name Maria Josefina Castex
Organization name Freiburg Uniklinik
Street address BreisacherStrasse 66
City Freiburg
ZIP/Postal code 79106
Country Germany
Platform ID GPL17021
Series (2)
GSE78264 Lsd1 ChIP-Seq in trophoblast stem cells
GSE78782 Deletion of Lsd1 triggers Senescence in Trophoblast Stem Cells by Induction of Sirt4
BioSample SAMN04513612
SRA SRX1598421

Supplementary file Size Download File type/resource
GSM2070601_TSC_Lsd1_peaks.bed.gz 211.3 Kb (ftp)(http) BED
GSM2070601_TSC_Lsd1_treat_afterfiting_all.wig.gz 638.9 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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