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Sample GSM2071327 Query DataSets for GSM2071327
Status Public on Mar 18, 2016
Title single cell RNA-seq from Purkinje cell (ENCLB921XPI)
Sample type SRA
 
Source name Mus musculus strain B6CASTF1/J Purkinje primary cell male adult (14 months)
Organism Mus musculus
Characteristics cell_type: Purkinje cell
biomaterial_type: primary cell
description: Mm_Castaneus X C57Bl_singlecell_male_parasagittal_Purkinje
biosample encode accession: ENCBS113OCQ (SAMN04284008)
age: 14 month
donor_id: ENCDO509HIY
dev stage: adult
Sex: male
lab: Barbara Wold, Caltech
link: ENCBS113OCQ at ENCODE; https://www.encodeproject.org/ENCBS113OCQ/
link: Derived from ENCODE donor ENCDO509HIY; https://www.encodeproject.org/ENCDO509HIY/
link: general protocol; https://www.encodeproject.org/documents/a6e06058-5019-488f-9195-8bcd2e9229c1/@@download/attachment/Laser%20capture%20dissection%20procedure%20general%20protocol_Wold_group.pdf
Extracted molecule total RNA
Extraction protocol general protocol: https://www.encodeproject.org/documents/5f5193b0-0c61-40c6-a286-b1f6d6f0c0da/@@download/attachment/SmartSeqProtocol.pdf
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description https://www.encodeproject.org/experiments/ENCSR111QQX/
***************
biological replicate number: 1
technical replicate number: 1
description: RNA-seq of a single Purkinje cell from a 14 month old male hybrid Castaneus X C57Bl6 mouse
experiment encode accession: ENCSR111QQX
assay title: single cell RNA-seq
assembly: mm10
possible controls: ENCSR620HJQ
encode release date: 2015-10-13
lab: Barbara Wold, Caltech
library encode accession: ENCLB921XPI
size range: >200
spikeins used: /references/ENCSR013YHQ/, /references/ENCSR133ALU/
nucleic acid starting quantity: 1.0
nucleic acid starting quantity units: cells
Data processing See GSM*_README.txt supplementary file linked below
 
Submission date Feb 24, 2016
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL13112
Series (1)
GSE78328 single cell RNA-seq from Purkinje cell (ENCSR111QQX)
Relations
SRA SRX1603027
BioSample SAMN04284008
Named Annotation GSM2071327_ENCFF101ZPW_signal_of_all_reads_mm10.bigWig
Named Annotation GSM2071327_ENCFF748MVT_signal_of_unique_reads_mm10.bigWig

Supplementary file Size Download File type/resource
GSM2071327_ENCFF101ZPW_signal_of_all_reads_mm10.bigWig 78.1 Mb (ftp)(http) BIGWIG
GSM2071327_ENCFF213KZG_gene_quantifications_mm10.tsv.gz 1.9 Mb (ftp)(http) TSV
GSM2071327_ENCFF748MVT_signal_of_unique_reads_mm10.bigWig 52.1 Mb (ftp)(http) BIGWIG
GSM2071327_ENCFF932YVH_transcript_quantifications_mm10.tsv.gz 3.9 Mb (ftp)(http) TSV
GSM2071327_ENCLB921XPI_README.txt.gz 646 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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