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Sample GSM2071400 Query DataSets for GSM2071400
Status Public on Mar 18, 2016
Title single cell RNA-seq from Purkinje cell (ENCLB350NUB)
Sample type SRA
 
Source name Homo sapiens Purkinje primary cell male adult (20 years)
Organism Homo sapiens
Characteristics cell_type: Purkinje cell
biomaterial_type: primary cell
description: Single Purkinje cell from a 20 year old male brain, part of a set of 20 cells
biosample encode accession: ENCBS982VXC (SAMN04284509)
age: 20 year
donor_id: ENCDO377OGA
dev stage: adult
Sex: male
health state: normal
lab: Barbara Wold, Caltech
link: ENCBS982VXC at ENCODE; https://www.encodeproject.org/ENCBS982VXC/
link: Derived from ENCODE donor ENCDO377OGA; https://www.encodeproject.org/ENCDO377OGA/
Extracted molecule total RNA
Extraction protocol data sheet: https://www.encodeproject.org/documents/28fef8e0-e171-450d-b66d-e3d199531cf2/@@download/attachment/Ambion-ERCC-Mix1.pdf
extraction method: None
general protocol: https://www.encodeproject.org/documents/a6e06058-5019-488f-9195-8bcd2e9229c1/@@download/attachment/Laser%20capture%20dissection%20procedure%20general%20protocol_Wold_group.pdf
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description https://www.encodeproject.org/experiments/ENCSR366QZI/
***************
biological replicate number: 1
technical replicate number: 1
description: RNA-seq of a single Purkinje cell from a 20 year old human male
experiment encode accession: ENCSR366QZI
assay title: single cell RNA-seq
assembly: GRCh38, hg19
possible controls: ENCSR888LYA
encode release date: 2014-07-07
lab: Barbara Wold, Caltech
library encode accession: ENCLB350NUB
size range: >200
spikeins used: /references/ENCSR013YHQ/, /references/ENCSR133ALU/
nucleic acid starting quantity: 1
nucleic acid starting quantity units: cells
Data processing See GSM*_README.txt supplementary file linked below
 
Submission date Feb 24, 2016
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL11154
Series (1)
GSE78376 single cell RNA-seq from Purkinje cell (ENCSR366QZI)
Relations
SRA SRX1603098
BioSample SAMN04284509
Named Annotation GSM2071400_ENCFF394YJU_signal_of_unique_reads_GRCh38.bigWig
Named Annotation GSM2071400_ENCFF487OJE_signal_of_all_reads_GRCh38.bigWig

Supplementary file Size Download File type/resource
GSM2071400_ENCFF161DUF_signal_of_unique_reads_hg19.bigWig 34.4 Mb (ftp)(http) BIGWIG
GSM2071400_ENCFF215IQA_signal_of_unique_reads_hg19.bigWig 36.1 Mb (ftp)(http) BIGWIG
GSM2071400_ENCFF287OET_transcript_quantifications_hg19.tsv.gz 7.0 Mb (ftp)(http) TSV
GSM2071400_ENCFF394YJU_signal_of_unique_reads_GRCh38.bigWig 36.1 Mb (ftp)(http) BIGWIG
GSM2071400_ENCFF417AIX_signal_of_all_reads_hg19.bigWig 39.8 Mb (ftp)(http) BIGWIG
GSM2071400_ENCFF484EKO_gene_quantifications_hg19.tsv.gz 2.5 Mb (ftp)(http) TSV
GSM2071400_ENCFF487OJE_signal_of_all_reads_GRCh38.bigWig 42.9 Mb (ftp)(http) BIGWIG
GSM2071400_ENCFF791NFX_signal_of_all_reads_hg19.bigWig 42.4 Mb (ftp)(http) BIGWIG
GSM2071400_ENCFF792DQG_transcript_quantifications_GRCh38.tsv.gz 7.1 Mb (ftp)(http) TSV
GSM2071400_ENCFF942SYY_gene_quantifications_GRCh38.tsv.gz 2.6 Mb (ftp)(http) TSV
GSM2071400_ENCLB350NUB_README.txt.gz 982 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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