|
| Status |
Public on Oct 10, 2007 |
| Title |
dif1_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
stage 14 ovules
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Ecotype: Columbia
1 month old plant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from dissected uvules by RNAqueous -micro spin columns (Ambion)
|
| Label |
biotin
|
| Label protocol |
Oligo-DT selection, Reverse transcription, second strand synthesis, biotin labeling (Bioprime kit)
|
| |
|
| Hybridization protocol |
hybridization protocol for eukaryotic cRNA target (Affymetrix)
|
| Scan protocol |
Chips were scanned by an Affymetrix Scanner 7G with GeneChip Operational System (Affymetrix)
|
| Description |
total RNA from dif1 ovules
|
| Data processing |
bg.adjust and quantile.normalize functions of affy R package were used on PM values
|
| |
|
| Submission date |
Jul 05, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Matthew Jones-Rhoades |
| E-mail(s) |
mjonesrh@bsd.uchicago.edu
|
| Organization name |
University of Chicago
|
| Street address |
W519, 929 E. 57th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1979 |
| Series (1) |
| GSE8392 |
Genome-Wide Expression Profiling of the Arabidopsis Female Gametophyte Identifies Families of Small, Secreted Proteins |
|
