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Sample GSM2086256 Query DataSets for GSM2086256
Status Public on May 12, 2017
Title bonobo_101116_lane_6
Sample type SRA
Source name placenta
Organism Pan paniscus
Characteristics tissue: placenta
Extracted molecule total RNA
Extraction protocol The villous portion of the tissue was homogenized in Trizol resulting in the isolation of total RNA following the manufacturer's instructions (Life Technologies, Grand Island, NY). The RNA was further purified using Qiagen’s RNeasy Midi Kit in conjunction with the RNase-Free DNase Set following the manufacturer’s protocol to remove any contaminating DNA from the sample (Valencia, CA)
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer II
Data processing Reads failing QC were removed using fastQC, aligned using STAR, and quantified using Cufflinks against appropriate ENSEMBL reference genomes
Genome_build: ENSEMBL v80
Supplementary_files_format_and_content: gene FPKM values from cufflinks
Submission date Mar 11, 2016
Last update date May 15, 2019
Contact name Don Leigh Armstrong
Organization name University of Illinois at Urbana Champaign
Department Institute for Genomic Biology
Lab Computing Genomes for Reproductive Health
Street address 1206 W Gregory Drive
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platform ID GPL21585
Series (1)
GSE79121 Expression Profiling of Term Placenta in Viviparous Mammals by RNA-Seq
BioSample SAMN04546399
SRA SRX1629202

Supplementary file Size Download File type/resource
GSM2086256_pan_paniscus_genes.fpkm_tracking.gz 766.1 Kb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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