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Sample GSM2096242 Query DataSets for GSM2096242
Status Public on Jun 01, 2017
Title 70181
Sample type protein
 
Source name Peripheral Blood
Organism Homo sapiens
Characteristics tissue: Serum
type: Healthy Control
array batch: GST1 - 4; FLAG
Extracted molecule protein
Extraction protocol Peripheral blood samples were drawn from the antecubital vein and serum was prepared according to a standardized protocol before freezing serum aliquots at -80oC
Label Serum samples are not labeled
Label protocol No lable was performed on the serum sample
 
Hybridization protocol Plasma hybridization was performed on TECAN HS 4800 Pro. 1. Protein arrays were blocked with 5% milk PBST (0.2% Tween) for 1 hour at RT; 2. 150ul 1:50 diluted serum was applied to each slide with medium agitation 16 hours at 4oC; 3. Slides were incubated with 150ul 1:500 dilution of Dylight 649-linked anti-human secondary antibodies (Jackson Immunoresearch) in milk PBST.
Scan protocol Slides were scanned by TECAN PowerScanner at 50% gain, 50% laser and 10┬Ám resolution.
Description The serum was applied on the protein array, secondary anti-human IgG antibody used for detection.
Data processing 1. The background signal estimated by the 25th percentile of the negative control; 2. Normalize the array by the median.
 
Submission date Mar 22, 2016
Last update date Jun 01, 2017
Contact name Benjamin A Katchman
Organization name Arizona State University
Street address 727 E. Tyler St.
City Tempe
State/province AZ
ZIP/Postal code 85287
Country USA
 
Platform ID GPL21622
Series (2)
GSE79498 Autoanitbody Biomarkers for the Early Detection of Serous Ovarian Cancer [Discovery Screen]
GSE79517 Autoanitbody Biomarkers for the Early Detection of Serous Ovarian Cancer

Data table header descriptions
ID_REF
VALUE Normalized signal

Data table
ID_REF VALUE
1 0.627823482
2 1.643422653
3 0.320195984
4 0.444766729
5 2.044024124
6 1.773730932
7 1.344553352
8 1.719760227
9 0.499164779
10 1.12237487
11 1.331325528
12 0.303553012
13 0.606506164
14 2.828392848
15 2.04092509
16 0.296247231
17 0.396554588
18 0.426779287
19 0.152097631
20 0.671698256

Total number of rows: 2352

Table truncated, full table size 34 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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