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Status |
Public on Oct 28, 2016 |
Title |
Ssb2 ChIP-exo, WT, Induced, Rep2 |
Sample type |
SRA |
|
|
Source name |
Yeast DNA
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
strain: DSB-PpoI system ppoi induction: ON antibody: a-Flag protein tag: Flag tag beads: a-Flag
|
Treatment protocol |
For treatment protocol please refer to the Materials and Methods.
|
Growth protocol |
Cultures were grown in full media (YEA) at 30ÂșC to O.D. of 0.8.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
For extraction and library preparation protocol please refer to the Materials and Methods. (ChIP-exo) For extraction and library preparation protocol please refer to the Materials and Methods. (ChIP-exo)
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|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Demultiplexing and removal of adapter conatmination was done in one step. Reads were mapped to modified S. pombe genome, with Bowtie2 software. (v2.2.5) PCR duplicates were filtered out and genome-wide coverage was calculated using R Bioconductor packages. Datasets were normalised for coverage over Chr I. Genome_build: ASM294v2.29 with insertion at position 1231078. Insert length is 1842 bases. Insert sequence (txt file), modified genome (fasta format) and modified annotations (bedfile) are included in the upload. Supplementary_files_format_and_content: BigWig format containing normalised genome-wide coverage for forward (.fw) and for reverse (.rev) strands separately
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|
|
Submission date |
Apr 18, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Tamas Fischer |
E-mail(s) |
tamas.fischer@bzh.uni-heidelberg.de
|
Phone |
+49 6221 544728
|
Organization name |
Biochemie Zentrum Heidelebrg
|
Department |
BZH
|
Lab |
AG Fischer
|
Street address |
Im Neuenheimer Feld 328
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL13988 |
Series (2) |
GSE80398 |
Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair |
GSE84883 |
Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair |
|
Relations |
BioSample |
SAMN04867750 |
SRA |
SRX1711764 |