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Sample GSM2127214 Query DataSets for GSM2127214
Status Public on Apr 14, 2021
Title PCOS Obesity 4
Sample type RNA
 
Source name PCOS Obesity Granulosa Cells
Organism Homo sapiens
Characteristics gender: female
age: 34 years old
menstrual cycle: 30-50 days
disease state: PCOS
weight: obese
cell type: Human Controlled Ovarian Stimulation Cycle Ovary Granulosa Cells
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy Fibrous Tissue kit (Qiagen) according to the manufacturer’s instructions.
Label biotin
Label protocol The Affymetrix Whole Transcript Sense Target Labeling Assay (Affymetrix) is used to create biotin – labeled sense -strand cDNA targets for hybridization to the Gene 1.0 ST Arrays.
 
Hybridization protocol Hybridization cocktail for a single probe array contained 2.5 pg of fragmented cRNA, 50 pM Control Oligonucleotide B2 (Affymetrix), 20X Eukaryotic Hybridization Controls (1.5 pM bioB, 5 pM bioC, 25 pM bioD, 100 pM cre) (Affymetrix), 7% DMSO, and 1X Hybridization Buffer. GeneChip was hybridized at 45℃ for 18 hours in the GeneChip Hybridization Oven 640 (Affymetrix)
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description A woman is 34 years old and whose menstrual cycle is 30-50 days.
GCS107277
Data processing The data were analyzed with GeneSpring version 7.3 using RMA normalization, then normalized to median of all samples.
 
Submission date Apr 19, 2016
Last update date Apr 14, 2021
Contact name Ruxing Wu
Organization name Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
Department Obstetrics and Gynecology
Street address No.1095 Jie Fang Avenue, Hankou, Wuhan,Hubei, China.
City Wuhan
State/province Hubei
ZIP/Postal code 430030
Country China
 
Platform ID GPL6244
Series (1)
GSE80432 Different gene expression profiles of granulosa cells in Chinese obese patients with or without polycystic ovary syndrome

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
7896736 6.194223
7896738 3.277655
7896740 3.087172
7896742 7.60045
7896744 5.172957
7896746 9.800221
7896748 7.492752
7896750 4.44152
7896752 10.657351
7896754 6.338375
7896756 5.379102
7896759 5.570434
7896761 6.742588
7896779 6.950889
7896798 6.919716
7896817 6.879691
7896822 6.696075
7896859 6.029433
7896861 3.983018
7896863 5.246912

Total number of rows: 28829

Table truncated, full table size 476 Kbytes.




Supplementary file Size Download File type/resource
GSM2127214_GCS107277.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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