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Sample GSM2127602 Query DataSets for GSM2127602
Status Public on Feb 18, 2019
Title Rosa males
Sample type SRA
Source name Kidney
Organism Mus musculus
Characteristics bmpr2 mutationtype: Rosa
treatment: Control
gender: male
Extracted molecule polyA RNA
Extraction protocol RNA was harvested using a Qiagen RNEasy kit with 1 ug of total RNA used for the construction of the sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols for stranded libraries
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description flash frozen kidney from 3 mice pooled
Data processing alignment with STAR v 2.5.1b, controlling for unannotated, non-canonical and rare junctions
read count generation during STAR alignment using –quantmode GeneCounts option that works like HTSeq-Count in default mode
Genome_build: Bos taurus genome build UMD3.1
Supplementary_files_format_and_content: txt files, raw read counts output from STAR alignment using option -–quantmode GeneCounts
Submission date Apr 19, 2016
Last update date May 15, 2019
Contact name James West
Organization name Vanderbilt University
Department Medicine
Lab Allergy, Pulmonary and Critical Care
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
Platform ID GPL21493
Series (1)
GSE80449 Effect of estrogens and BMPR2 on mouse kidney gene expression
BioSample SAMN04874678
SRA SRX1715822

Supplementary file Size Download File type/resource 262.1 Kb (ftp)(http) TAB
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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