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Sample GSM2128751 Query DataSets for GSM2128751
Status Public on Jan 01, 2017
Title 2H 128μg/ml GR-SU_vs_control_3
Sample type RNA
 
Channel 1
Source name S.aureus 2H 128μg/ml GR-SU
Organism Staphylococcus aureus subsp. aureus MW2
Characteristics strain: MW2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the bacterial cells using a FastRNA Pro Blue Kit (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Test and control cDNAs were labelled with Cy3 and Cy5 (GE Healthcare UK), respectively.
 
Channel 2
Source name S.aureus 2H 0μg/ml GR-SU
Organism Staphylococcus aureus subsp. aureus MW2
Characteristics strain: MW2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the bacterial cells using a FastRNA Pro Blue Kit (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer’s protocol.
Label Cy5
Label protocol Test and control cDNAs were labelled with Cy3 and Cy5 (GE Healthcare UK), respectively.
 
 
Hybridization protocol 65℃ 20H
Scan protocol The arrays were then scanned with an Agilent scanner (Agilent Technologies).
Description Gene expression after 2H
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Apr 20, 2016
Last update date Jan 01, 2017
Contact name Kentaro Oyama
Organization name Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
Street address Sakuragaoka 8-35-1
City Kagoshima City
ZIP/Postal code 890-8544
Country Japan
 
Platform ID GPL21747
Series (1)
GSE80500 Effect of Glycyrrhetinic acid and its derivatives on antibacterial activity and virulence against Staphylococcus aureus

Data table header descriptions
ID_REF
VALUE log10 Cy3/Cy5 representing reference/test
INV_VALUE log10 Cy5/Cy3 representing reference/test

Data table
ID_REF VALUE INV_VALUE
1 -0.965515 0.965515472
2 0.500798 -0.500798282
3 0.487956 -0.48795552
4 -0.165749 0.165748664
5 -0.183859 0.183858858
6 0.100037 -0.100036735
7 -0.174734 0.174734151
8 0.22814 -0.228139688
9 0.0309386 -0.030938637
10 0.063561 -0.063560995
11 0.12458 -0.124580321
12 -0.0412661 0.041266092
13 -0.0656004 0.06560043
14 -0.288009 0.288008701
15 0.572636 -0.572635934
16 -0.270477 0.270476693
17 -0.0816627 0.081662688
18 -0.2685 0.268500106
19 -0.0262899 0.026289864
20 -0.193705 0.193704945

Total number of rows: 15643

Table truncated, full table size 419 Kbytes.




Supplementary file Size Download File type/resource
GSM2128751_US11030397_256409610007_S01_GE2_107_Sep09_1_3.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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