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Sample GSM2128752 Query DataSets for GSM2128752
Status Public on Jan 01, 2017
Title 2H 128μg/ml GR-SU_vs_control_4
Sample type RNA
 
Channel 1
Source name S.aureus 2H 128μg/ml GR-SU
Organism Staphylococcus aureus subsp. aureus MW2
Characteristics strain: MW2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the bacterial cells using a FastRNA Pro Blue Kit (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Test and control cDNAs were labelled with Cy3 and Cy5 (GE Healthcare UK), respectively.
 
Channel 2
Source name S.aureus 2H 0μg/ml GR-SU
Organism Staphylococcus aureus subsp. aureus MW2
Characteristics strain: MW2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the bacterial cells using a FastRNA Pro Blue Kit (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer’s protocol.
Label Cy5
Label protocol Test and control cDNAs were labelled with Cy3 and Cy5 (GE Healthcare UK), respectively.
 
 
Hybridization protocol 65℃ 20H
Scan protocol The arrays were then scanned with an Agilent scanner (Agilent Technologies).
Description Gene expression after 2H
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Apr 20, 2016
Last update date Jan 01, 2017
Contact name Kentaro Oyama
Organization name Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
Street address Sakuragaoka 8-35-1
City Kagoshima City
ZIP/Postal code 890-8544
Country Japan
 
Platform ID GPL21747
Series (1)
GSE80500 Effect of Glycyrrhetinic acid and its derivatives on antibacterial activity and virulence against Staphylococcus aureus

Data table header descriptions
ID_REF
VALUE log10 Cy3/Cy5 representing reference/test
INV_VALUE log10 Cy5/Cy3 representing reference/test

Data table
ID_REF VALUE INV_VALUE
1 -1.26742 1.267417591
2 0.488262 -0.488262424
3 0.485715 -0.485714808
4 -0.180453 0.180452633
5 -0.271472 0.271471913
6 -0.128267 0.128266722
7 -0.189388 0.189387653
8 0.182683 -0.182682984
9 0.0325051 -0.032505072
10 -0.127914 0.127913664
11 0.0484981 -0.048498098
12 -0.0531033 0.053103253
13 -0.297722 0.297721677
14 -0.244915 0.244914653
15 0.355005 -0.3550047
16 -0.275839 0.275838803
17 -0.0118066 0.011806588
18 -0.140327 0.140326515
19 0.0261432 -0.026143162
20 -0.278772 0.278772263

Total number of rows: 15643

Table truncated, full table size 420 Kbytes.




Supplementary file Size Download File type/resource
GSM2128752_US11030397_256409610007_S01_GE2_107_Sep09_1_4.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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