|
| Status |
Public on Apr 22, 2016 |
| Title |
CDCP1+TICs untreated 24h rep2 [FatK7_C2] |
| Sample type |
RNA |
| |
|
| Source name |
CDCP1+TICs untreated 24h
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
genotype/variation: Fapbl-Cre;Apc2lox14/+;tet-KrasLSL-G12D/+(FatK)
mouse id: mice#7
age: 17 weeks
tissue: Colon
cell type: CDCP1+ tumor inducing cells (TICs)
treated with: none (untreated)
|
| Treatment protocol |
CDCP1+ cells isolated from primary FatK1, FatK5 and FatK7 tumors were maintained with Dox and treated with or without Foxd3 shRNA for 24h
|
| Growth protocol |
When magnetic-activated cell sorting (MACS) was used to sort for CDCP1+ cells, dissociated tumor cells from FatK1,FatK5,FatK7 mice were incubated with a monoclonal CDCP1 antibody labeled with MicroBeads (Miltenyi Biotec) for 30 min at 4℃, followed by cleavage of the MicroBeads.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
FatK7_C2
|
| Data processing |
The data were normalised using global median normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 21, 2016 |
| Last update date |
Apr 22, 2016 |
| Contact name |
Li Qingquan |
| E-mail(s) |
061101040@fudan.edu.cn
|
| Organization name |
fudan university
|
| Street address |
No.138，Yixueyuan Rd.
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE80505 |
Genome-wide analysis of differential gene expression after Foxd3 knockdown in colorectal CDCP1+CSCs |
|
