|
| Status |
Public on Aug 01, 2018 |
| Title |
HPV 16 positive and HIV negative cases Sample1 |
| Sample type |
RNA |
| |
|
| Source name |
Human cervical smear collected with cyto brush
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Cervical cells
gender: female
age: 60 years
|
| Treatment protocol |
The cervical cells were pelleted down at approximately 6000 rpm and used for isolating Total RNA
|
| Growth protocol |
The Cervical cells were collected from the subjects in preservecyt medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Ambion MiRVAna Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The RNA samples were labeled with the Genisphere FlashTag Biotin HSR RNA labeling kit (Genisphere, Hatfield, United Kingdom).
|
| |
|
| Hybridization protocol |
The labeled RNA was quantified, fractionated and hybridized to the miRNA microarray according to the standard procedures provided by the manufacture.The chips were washed and stained using a Genechip Fluidics Station 450 (Affymetrix, Santa Clara, California, United States).
|
| Scan protocol |
The chips were then scanned with an Affymetrix GeneChip Scanner 3000 (Affymetrix, Santa Clara, California, United States).
|
| Description |
microRNA expression from human cervical cells
|
| Data processing |
The Data generated from the scan was then analyzed using the Affymetrix GeneChip® Command Console™ Software, v1.1, and the Affymetrix GeneChip® Expression Console software, v 1.1
|
| |
|
| Submission date |
May 05, 2016 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Radhakrishna Madhavan Pillai |
| Organization name |
Rajiv Gandhi Centre for Biotechnology
|
| Department |
Cancer Research Program
|
| Lab |
CRP-9
|
| Street address |
poojappura
|
| City |
trivandrum |
| State/province |
KERALA |
| ZIP/Postal code |
695014 |
| Country |
India |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE81137 |
microRNA expression profiling in HPV-16/HIV associated Cervical Cancer |
|
