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Sample GSM2143658 Query DataSets for GSM2143658
Status Public on Jun 12, 2016
Title SGC_H4K5ac_ChIP
Sample type SRA
Source name MV4;11 cell line
Organism Homo sapiens
Characteristics tissue: peripheral blood
cell line: MV4;11
treatment: SGC0946
chip antibody: H4K5ac
Treatment protocol Cell lines were grown in 0.1% DMSO or 5uM of SGC0946
Growth protocol Cell lines were grown in RPMI-1640 supplemented with 20% fetal calf serum, penicillin (100 units/mL) and streptomycin (100 ug/mL)
Extracted molecule genomic DNA
Extraction protocol Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody
ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Data processing Illumina Casava1.8.2 software was used for basecalling.
Reads were aligned to the human genome (G1k V37) using bwa 0.6.2-r126
Peak-calling was performed using MACS2
Genome_build: hg19
Supplementary_files_format_and_content: bed files from MACS2 for each sample
Submission date May 05, 2016
Last update date May 15, 2019
Contact name Mark Dawson
Organization name Peter MacCallum Cancer Centre
Street address 305 Grattan Street
City Melbourne
State/province VIC
ZIP/Postal code 3000
Country Australia
Platform ID GPL11154
Series (2)
GSE71776 ChIPSeq of MV4;11 cell treated with DMSO, I-BET, SGC0946 and combination of I-BET and SGC0946
GSE71780 Functional interdependency of BRD4 and DOT1L in MLL leukaemia
BioSample SAMN04956439
SRA SRX1743599

Supplementary file Size Download File type/resource
GSM2143658_K5ac96hr_peaks.bed.gz 1.1 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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