|
| Status |
Public on Aug 01, 2008 |
| Title |
18S z000509 KLF4-2 CY5 vs STD-2 CY3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
klf4 MO
|
| Organism |
Danio rerio |
| Characteristics |
Total RNA from pooled 18S Fertilized embryos from natural matings injected with a klf4 specific antisense morpholino oligonucleotide (klf4-MO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAAmplification Ambion
|
| Label |
Cy5
|
| Label protocol |
Indirect aRNA Ambion
|
| |
|
| Channel 2 |
| Source name |
STD MO
|
| Organism |
Danio rerio |
| Characteristics |
Total RNA from pooled 18S Fertilized embryos from natural matings injected with a standard nonsense morpholino oligonucleotide (Std-MO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAAmplification Ambion
|
| Label |
Cy3
|
| Label protocol |
Indirect aRNA Ambion
|
| |
|
| |
| Hybridization protocol |
Followed protocol described in:
PMID: 15998744
|
| Scan protocol |
Followed protocol described in:
PMID: 15998744
|
| Description |
Pairwise comparison of STD-MO vs klf4-MO
|
| Data processing |
Imagene5.6, Lowess Normalisation (R)
|
| |
|
| Submission date |
Aug 02, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Darrin F Taylor |
| E-mail(s) |
d.taylor@imb.uq.edu.au
|
| Phone |
+61 7 3346 2607
|
| Organization name |
Institute for Molecular Bioscience, Uni of Qld
|
| Department |
Genomics and Bioinformatics
|
| Lab |
Expression Genomics Lab
|
| Street address |
Research Rd
|
| City |
St Lucia |
| State/province |
Qld |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL5675 |
| Series (1) |
| GSE8656 |
Pair wise comparison between STD-MO and klf4-MO embryos |
|
