|
| Status |
Public on Feb 16, 2017 |
| Title |
N2 #1 RNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
C. elegans
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2
developmental stage: Young adult
|
| Growth protocol |
Synchronized to young adults by timed egg lay
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Trizol/chroloroform, isopropanol precipitation, DNaseI treatment, purification and concentration
Illumina TruSeq RNA Library Preparation Kit v2
(In SAMPLES section)
(In SAMPLES section)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
N2_HC125_RNA-seq_PairedEnd_gene_exp_diff.xlsx
Paired end, no strand information
|
| Data processing |
Align to WS235 with Tophat v. 2.0.1; -i 20, -I 10000 and all other parameters set to default
Identify differentially expressed genes with Cufflinks v. 2.2.1; default parameters; FDR < .05
Genome_build: C. elegans WS235
|
| |
|
| Submission date |
May 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Olga Minkina |
| E-mail(s) |
ominkina@gmail.com
|
| Organization name |
Harvard University
|
| Department |
Molecular and Cellular Biology
|
| Lab |
Craig P. Hunter
|
| Street address |
16 Divinity Ave.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL19757 |
| Series (2) |
| GSE81707 |
Germline- and soma-specific heritable epigenetic silencing at an endogenous locus (RNA-Seq) |
| GSE81708 |
Germline- and soma-specific heritable epigenetic silencing at an endogenous locus |
|
| Relations |
| BioSample |
SAMN05157451 |
| SRA |
SRX1787479 |
