|
Status |
Public on Feb 16, 2017 |
Title |
N2 #2 RNA-seq |
Sample type |
SRA |
|
|
Source name |
C. elegans
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: N2 developmental stage: Young adult
|
Growth protocol |
Synchronized to young adults by timed egg lay
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Trizol/chroloroform, isopropanol precipitation, DNaseI treatment, purification and concentration Illumina TruSeq RNA Library Preparation Kit v2 (In SAMPLES section) (In SAMPLES section)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
N2_HC125_RNA-seq_PairedEnd_gene_exp_diff.xlsx Paired end, no strand information
|
Data processing |
Align to WS235 with Tophat v. 2.0.1; -i 20, -I 10000 and all other parameters set to default Identify differentially expressed genes with Cufflinks v. 2.2.1; default parameters; FDR < .05 Genome_build: C. elegans WS235
|
|
|
Submission date |
May 20, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Olga Minkina |
E-mail(s) |
ominkina@gmail.com
|
Organization name |
Harvard University
|
Department |
Molecular and Cellular Biology
|
Lab |
Craig P. Hunter
|
Street address |
16 Divinity Ave.
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
|
|
Platform ID |
GPL19757 |
Series (2) |
GSE81707 |
Germline- and soma-specific heritable epigenetic silencing at an endogenous locus (RNA-Seq) |
GSE81708 |
Germline- and soma-specific heritable epigenetic silencing at an endogenous locus |
|
Relations |
BioSample |
SAMN05157453 |
SRA |
SRX1787481 |