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Sample GSM2171670 Query DataSets for GSM2171670
Status Public on Feb 16, 2017
Title N2 #3 RNA-seq
Sample type SRA
Source name C. elegans
Organism Caenorhabditis elegans
Characteristics strain: N2
developmental stage: Young adult
Growth protocol Synchronized to young adults by timed egg lay
Extracted molecule polyA RNA
Extraction protocol Trizol/chroloroform, isopropanol precipitation, DNaseI treatment, purification and concentration
Apollo 324™ NGS Library Prep System
(In SAMPLES section)
(In SAMPLES section)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description N2_HC125_RNA-seq_SingleEnd_gene_exp_diff.xlsx
Single end, stranded
Data processing Align to WS235 with Tophat v. 2.0.1; -i 20, -I 10000 and all other parameters set to default
Identify differentially expressed genes with Cufflinks v. 2.2.1; default parameters; FDR < .05
Genome_build: C. elegans WS235
Submission date May 20, 2016
Last update date May 15, 2019
Contact name Olga Minkina
Organization name Harvard University
Department Molecular and Cellular Biology
Lab Craig P. Hunter
Street address 16 Divinity Ave.
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platform ID GPL19757
Series (2)
GSE81707 Germline- and soma-specific heritable epigenetic silencing at an endogenous locus (RNA-Seq)
GSE81708 Germline- and soma-specific heritable epigenetic silencing at an endogenous locus
BioSample SAMN05157455
SRA SRX1787483

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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