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Sample GSM2174921 Query DataSets for GSM2174921
Status Public on Oct 23, 2018
Title M-1_BS
Sample type SRA
 
Source name normal mouse M-1 cells - bisulfite-treated mRNAs
Organism Mus musculus
Characteristics cell line: M-1
cell type: Collecting Tubular Epithelial
Extracted molecule total RNA
Extraction protocol mRNAs of of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement.
Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA)
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 3000
 
Description bisulfite treated mRNA
Mouse Collecting Tubular Epithelial
Data processing Raw reads for each sample were stripped of adaptor sequences and low quality bases were removed using Trimmomatic. Filtered paired-end reads were mapped against human (hg19) or mouse (mm10) reference genome by Bismark.
The methylated sites were called by Bismark (http://www.bioinformatics.babraham.ac.uk/projects/bismark/).
Genome_build: hg19 or mm10
Supplementary_files_format_and_content: supplementary file includes the methylated sites
 
Submission date May 23, 2016
Last update date May 15, 2019
Contact name Bao-Fa Sun
E-mail(s) sunbf@big.ac.cn
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
 
Platform ID GPL21493
Series (1)
GSE74771 Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome
Relations
BioSample SAMN05163474
SRA SRX1792778

Supplementary file Size Download File type/resource
GSM2174921_M-1_BS.txt.gz 133.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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