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Sample GSM2176248 Query DataSets for GSM2176248
Status Public on Oct 23, 2018
Title ALYREF-IP_rep2BS
Sample type SRA
Source name ALYREF-IP human HeLa cells - bisulfite-treated mRNAs
Organism Homo sapiens
Characteristics cell line: HeLa
cell type: Carcinoma cervical
Extracted molecule total RNA
Extraction protocol mRNAs of of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement.
Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA)
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 3000
Description bisulfite treated ALYREF-IP-mRNA
Human HeLa cells
Data processing Raw reads for each sample were stripped of adaptor sequences and low quality bases were removed using Trimmomatic. Filtered paired-end reads were mapped against human (hg19) or mouse (mm10) reference genome by Bismark.
The methylated sites were called by Bismark (
Genome_build: hg19
Supplementary_files_format_and_content: supplementary file includes the methylated sites
Submission date May 24, 2016
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL21290
Series (1)
GSE74771 Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome
BioSample SAMN05171360
SRA SRX1796307

Supplementary file Size Download File type/resource
GSM2176248_ALYREF-IP_rep2BS.txt.gz 137.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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