|
| Status |
Public on Aug 01, 2016 |
| Title |
LTAD siSOCS2-AS1 DHT |
| Sample type |
RNA |
| |
|
| Source name |
LTAD cells treated with DHT 10 nM 48h after siSOCS2-AS1 transfection.
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LTAD
cell type: prostate cancer
sirna: siSOCS2-AS1
ligand: DHT 10 nM
|
| Treatment protocol |
Cells were transfected with each siRNA 48 h before hormone treatment. Cells were treated with vehicle or DHT (10 nM).
|
| Growth protocol |
LNCaP cells were cultures in RPMI 1640 containing 10% FBS. LTAD cells were cultured in phenol red free RPMI containing 10% charcoal/dextran treated FBS. Before hormone treatment, cells were incubated in Phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using RNeasy (QIAGEN) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled employing the Affymetrix GeneChip Whole Transcript (WT) Terminal Labeling kit according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
The samples were hybridized to Human Gene 1.0 ST microarrays according to standardized protocols from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from LTAD cells treated with DHT 10 nM 48h after siSOCS2-AS1 transfection.
|
| Data processing |
The microarray data were preprocessed with Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
|
| |
|
| Submission date |
Jun 03, 2016 |
| Last update date |
Aug 01, 2016 |
| Contact name |
Ken-ichi Takayama |
| Organization name |
Tokyo Metropolitan Institute of Gerontology
|
| Street address |
Sakaecho
|
| City |
Itabashi-ku |
| ZIP/Postal code |
173-0015 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE82224 |
Effects of SOCS2-AS1 inhibition in prostate cancer cells |
| GSE82225 |
Androgen-induced lncRNA SOCS2-AS1 Promotes Cell Growth and Inhibits Apoptosis in Prostate Cancer Cells |
|
