|
| Status |
Public on Aug 22, 2007 |
| Title |
Healthy5-CD4 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood CD4 T cells
|
| Organism |
Homo sapiens |
| Characteristics |
Healthy
|
| Treatment protocol |
CD4 or CD8 T cells were isolated using negative selection magnetic labeled antibody mix.
|
| Growth protocol |
T cells were isolated from peripheral blood.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for overnight at 45C on Affymetrix hu133A microarray chip.
|
| Scan protocol |
Each cRNA bound at its complementary oligonucleotide was excited using confocal laser scanner, and the positions and intensities of the fluorescent emissions were captured. These measures provided the basis of subsequent analysis.
|
| Description |
peripheral blood CD4 T cells: Healthy
|
| Data processing |
The data were analysed using dCHIP (C Li and W Wong, Proc. Natl. Acad. Sci. Vol. 98, 31-36, 2001a. ).
|
| |
|
| Submission date |
Aug 21, 2007 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Gullu Gorgun |
| E-mail(s) |
gullu_gorgun@dfci.harvard.edu
|
| Phone |
617 632 3358
|
| Fax |
617 632 3222
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Street address |
44 Binney Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE8835 |
Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells. |
|
| Relations |
| Reanalyzed by |
GSE86363 |
