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Sample GSM2202923 Query DataSets for GSM2202923
Status Public on Aug 10, 2016
Title S2A/S7A_rep4
Sample type RNA
 
Channel 1
Source name WT
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: WT
Growth protocol Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
Label Cy5
Label protocol Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
Channel 2
Source name S2AS7A
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: rpb1-S2AS7A 18 repeats 'rump' + 14x YAPTSPA
Growth protocol Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
Label Cy3
Label protocol Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
 
Hybridization protocol Labelled cDNA was hybridized according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
Scan protocol Arrays were scanned on GenePix 4000B scanner and images were quantified GenePic Pro 6.1 software
Description Biological replicate 4 of 5, dye flip
Data processing Background subtraction and lowess normalization was applied according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
Submission date Jun 16, 2016
Last update date Aug 10, 2016
Contact name Jeffrey A Pleiss
E-mail(s) jpleiss@cornell.edu
Organization name Cornell University
Department Molecular Biology and Genetics
Street address 451 Biotech
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platform ID GPL21587
Series (1)
GSE83426 Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing mutant/WT control (unless dye flipped)

Data table
ID_REF VALUE
SPSNORNA.04_1 - ncRNA -0.68226832
SPSNORNA.24_1 - ncRNA -0.207834955
SPSNORNA.27_1 - ncRNA -0.119948341
SPSNORNA.11_1 - ncRNA -0.336905145
SPBTRNAGLY.09_1 - ncRNA 0.064591556
SPSNRNA.04_1 - ncRNA
SPSNORNA.19_1 - ncRNA -0.236479461
SPBTRNAARG.05_1 - ncRNA 0.052406981
SPSNORNA.15_1 - ncRNA -0.473236697
SPCTRNAARG.08_1 - ncRNA 0.002667844
SPATRNAARG.01_1 - ncRNA -0.072957556
SPBTRNAARG.04_1 - ncRNA -0.079584446
SPBTRNALEU.05_1 - ncRNA 0.10131468
SPCTRNAGLY.12_1 - ncRNA 0.040657355
SPSNORNA.06_1 - ncRNA -0.434126812
SPSNORNA.05_1 - ncRNA -0.286282917
SPATRNAMET.02_1 - ncRNA
SPATRNAILE.02_1 - ncRNA -0.514556662
SPATRNALYS.02_1 - ncRNA -0.039378125
SPSNORNA.02_1 - ncRNA -0.386122174

Total number of rows: 15207

Table truncated, full table size 514 Kbytes.




Supplementary file Size Download File type/resource
GSM2202923_Array2_2_WT_S2AS7A.gpr.gz 1.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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