|
| Status |
Public on Aug 10, 2016 |
| Title |
S7A_rep4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
S7A
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype/variation: rpb1-S7A 18 repeats 'rump' + 14x YSPTSPA
|
| Growth protocol |
Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
|
| Label |
Cy5
|
| Label protocol |
Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
|
| |
|
| Channel 2 |
| Source name |
WT
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype/variation: WT
|
| Growth protocol |
Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
|
| Label |
Cy3
|
| Label protocol |
Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
|
| |
|
| |
| Hybridization protocol |
Labelled cDNA was hybridized according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
|
| Scan protocol |
Arrays were scanned on GenePix 4000B scanner and images were quantified GenePic Pro 6.1 software
|
| Description |
Biological replicate 4 of 4
|
| Data processing |
Background subtraction and lowess normalization was applied according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
|
| |
|
| Submission date |
Jun 16, 2016 |
| Last update date |
Aug 10, 2016 |
| Contact name |
Jeffrey A Pleiss |
| E-mail(s) |
jpleiss@cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Molecular Biology and Genetics
|
| Street address |
451 Biotech
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL21587 |
| Series (1) |
| GSE83426 |
Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast |
|
