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Sample GSM2202928 Query DataSets for GSM2202928
Status Public on Aug 10, 2016
Title S2A_rep4
Sample type RNA
 
Channel 1
Source name S2A
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: rpb1-S2A 18 repeats 'rump' + 14x YAPTSPS
Growth protocol Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
Label Cy5
Label protocol Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
Channel 2
Source name WT
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: WT
Growth protocol Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
Label Cy3
Label protocol Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
 
Hybridization protocol Labelled cDNA was hybridized according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
Scan protocol Arrays were scanned on GenePix 4000B scanner and images were quantified GenePic Pro 6.1 software
Description Biological replicate 4 of 4
Data processing Background subtraction and lowess normalization was applied according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
Submission date Jun 16, 2016
Last update date Aug 10, 2016
Contact name Jeffrey A Pleiss
E-mail(s) jpleiss@cornell.edu
Organization name Cornell University
Department Molecular Biology and Genetics
Street address 451 Biotech
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platform ID GPL21587
Series (1)
GSE83426 Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing mutant/WT control (unless dye flipped)

Data table
ID_REF VALUE
SPSNORNA.04_1 - ncRNA -0.394242674
SPSNORNA.24_1 - ncRNA -0.349685722
SPSNORNA.27_1 - ncRNA 0.176042526
SPSNORNA.11_1 - ncRNA -0.328632401
SPBTRNAGLY.09_1 - ncRNA -0.881439043
SPSNRNA.04_1 - ncRNA -0.515737806
SPSNORNA.19_1 - ncRNA -1.503522691
SPBTRNAARG.05_1 - ncRNA -0.783123885
SPSNORNA.15_1 - ncRNA -0.041598219
SPCTRNAARG.08_1 - ncRNA -1.013853313
SPATRNAARG.01_1 - ncRNA 0.130598339
SPBTRNAARG.04_1 - ncRNA -1.245686423
SPBTRNALEU.05_1 - ncRNA 0.28551471
SPCTRNAGLY.12_1 - ncRNA -0.498351647
SPSNORNA.06_1 - ncRNA -0.328300821
SPSNORNA.05_1 - ncRNA -0.394366938
SPATRNAMET.02_1 - ncRNA -0.157463033
SPATRNAILE.02_1 - ncRNA -1.272776396
SPATRNALYS.02_1 - ncRNA -2.746887759
SPSNORNA.02_1 - ncRNA -0.359293821

Total number of rows: 15207

Table truncated, full table size 533 Kbytes.




Supplementary file Size Download File type/resource
GSM2202928_Array2_7_S2A_WT.gpr.gz 1.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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