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Sample GSM2202929 Query DataSets for GSM2202929
Status Public on Aug 10, 2016
Title S7E_rep3
Sample type RNA
 
Channel 1
Source name WT
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: WT
Growth protocol Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
Label Cy5
Label protocol Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
Channel 2
Source name S7E
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: rpb1-S7E 18 repeats 'rump' + 14x YSPTSPE
Growth protocol Cells were innoculated at an OD~0.1 in 25mL YES media and collected by centrifugation at mid-log-phase growth (OD~0.5). Replicates indicate separately grown biological replicates
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Acid-phenol extraction followed by ethanol precipitation and resuspension in 50uL TE buffer
Label Cy3
Label protocol Total RNA was reverse transcribed and labelled according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
 
Hybridization protocol Labelled cDNA was hybridized according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
Scan protocol Arrays were scanned on GenePix 4000B scanner and images were quantified GenePic Pro 6.1 software
Description Biological replicate 3 of 3, dye flip
Data processing Background subtraction and lowess normalization was applied according to (Inada, M & Pleiss, JA. 2010, PMID 20946806)
 
Submission date Jun 16, 2016
Last update date Aug 10, 2016
Contact name Jeffrey A Pleiss
E-mail(s) jpleiss@cornell.edu
Organization name Cornell University
Department Molecular Biology and Genetics
Street address 451 Biotech
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platform ID GPL21587
Series (1)
GSE83426 Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing mutant/WT control (unless dye flipped)

Data table
ID_REF VALUE
SPSNORNA.04_1 - ncRNA -0.402410186
SPSNORNA.24_1 - ncRNA -0.396130499
SPSNORNA.27_1 - ncRNA -0.350041745
SPSNORNA.11_1 - ncRNA -0.224275167
SPBTRNAGLY.09_1 - ncRNA -0.151193195
SPSNRNA.04_1 - ncRNA -0.092523597
SPSNORNA.19_1 - ncRNA -0.070659925
SPBTRNAARG.05_1 - ncRNA -0.039270148
SPSNORNA.15_1 - ncRNA -0.036435927
SPCTRNAARG.08_1 - ncRNA -0.023597138
SPATRNAARG.01_1 - ncRNA 0.002633199
SPBTRNAARG.04_1 - ncRNA 0.002995653
SPBTRNALEU.05_1 - ncRNA 0.024929967
SPCTRNAGLY.12_1 - ncRNA 0.056019467
SPSNORNA.06_1 - ncRNA 0.057968576
SPSNORNA.05_1 - ncRNA 0.058401397
SPATRNAMET.02_1 - ncRNA 0.061213226
SPATRNAILE.02_1 - ncRNA 0.068489744
SPATRNALYS.02_1 - ncRNA 0.089210265
SPSNORNA.02_1 - ncRNA 0.096744736

Total number of rows: 15207

Table truncated, full table size 532 Kbytes.




Supplementary file Size Download File type/resource
GSM2202929_Array2_8_WT_S7E.gpr.gz 1.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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