|
Status |
Public on Jan 01, 2017 |
Title |
Vector-1 |
Sample type |
SRA |
|
|
Source name |
HepG2.2.15-vector cell
|
Organism |
Homo sapiens |
Characteristics |
cell line: HepG2.2.15 lentivirus: vector
|
Treatment protocol |
CD36-overexpression-lentivirus and Vector-lentivirus were transfected into HepG2.2.15 cells.
|
Growth protocol |
Modified Eagle Medium (MEM) containing 10% fetal calf serum and 500g of G418/ml.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cells by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Agilent) and kept at -80℃. The RNA with RIN >8.0 is right for cDNA library construction. Ion Proton Sequencing Protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Ion Torrent Proton |
|
|
Description |
Processed data file: All_Counts.txt
|
Data processing |
Base calling using Torrent Suite Software and Torrent Server. Reads filtering under criteria removing reads with 20% of the base quality lower than 13. Mapsplice v2.1.8 mapping to reference genome. Reads counting using HTSeq. Genome_build: hg19 (GRCh37) Supplementary_files_format_and_content: All_Counts.txt: Tab-delimited text file contains raw counts for each sample.
|
|
|
Submission date |
Jun 21, 2016 |
Last update date |
May 15, 2019 |
Contact name |
健 黄 |
E-mail(s) |
zmchj2001@163.com
|
Phone |
15086067610
|
Organization name |
遵义医学院附属医院
|
Street address |
汇川区大连路149号遵义医学院附属医院
|
City |
遵义市 |
State/province |
贵州省 |
ZIP/Postal code |
563003 |
Country |
China |
|
|
Platform ID |
GPL17303 |
Series (1) |
GSE83577 |
Genome-wide Transcriptome Analysis of CD36 Overexpression in HepG2.2.15 Cells to Explore Its Regulation Role of Metabolism and HBV Life Cycle |
|
Relations |
BioSample |
SAMN05277752 |
SRA |
SRX1868801 |