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Status |
Public on Jun 30, 2017 |
Title |
B_TotalDNA-seq_AkataZta+I-BET_Replicate3 |
Sample type |
SRA |
|
|
Source name |
Akata-Zta (EBV+ Burkitt lymphoma cell line)
|
Organisms |
Homo sapiens; human gammaherpesvirus 4 |
Characteristics |
cell line: Akata-Zta pathogen: Epstein-Barr virus 1h pretreatment: I-BET 1 day treatment: vehicle
|
Treatment protocol |
Log phase cultures were pretreated for 1 hour with 1 μM JQ1 (EMD Millipore), I-BET/GSK525762A (EMD Millipore), RVX-208 (Cayman Chemical), or vehicle, then treated for 1 day with 500 ng/mL doxycycline (Sigma-Aldrich) or vehicle
|
Growth protocol |
Akata-Zta cells were maintained in RPMI-1640 with 25 mM HEPES and 2 g/L NaHCO3 in 5% CO2 with 10% (v/v) Tet System Approved fetal bovine serum (Clontech)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
EBV genome abundance was determined by deep sequencing of total DNA. Input chromatin was prepared as described in the paper. Total DNA-seq libraries were prepared as described in the paper
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|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina HiSeq 4000 |
|
|
Data processing |
Library strategy: DNA-seq 50 bp reads were mapped using Bowtie to an index containing both the human hg19 and EBV reference [GenBank ID: NC_007605.1] genomes. Parameters allowed for up to two mismatches and only considered reads that mapped to a unique sequence. The percentage of EBV reads was calculated as a proportion of all reads that mapped EBV genome abundance upon lytic induction and/or drug pretreatment was normalized to the vehicle control in each individual set of experiments Genome_build: index contains both the human hg19 and EBV reference [GenBank ID: NC_007605.1] genomes Supplementary_files_format_and_content: Spreadsheet shows total reads, EBV reads, and EBV quantitation
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|
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Submission date |
Jul 10, 2016 |
Last update date |
May 15, 2019 |
Contact name |
JJ Miranda |
E-mail(s) |
jmiranda@barnard.edu
|
Organization name |
Barnard College, Columbia University
|
Street address |
3009 Broadway
|
City |
New York |
ZIP/Postal code |
10027 |
Country |
USA |
|
|
Platform ID |
GPL22132 |
Series (2) |
GSE84212 |
BET inhibitors suppress lytic DNA replication [Akata-Zta DNA-seq] |
GSE84214 |
BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps |
|
Relations |
BioSample |
SAMN05370844 |
SRA |
SRX1923360 |