|
| Status |
Public on Jun 12, 2017 |
| Title |
5_NEKO_male_238 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney_NEKO_male
|
| Organism |
Mus musculus |
| Characteristics |
strain background: Mixed (with contributions from 129Sv/J and C57BL/6J strains)
genotype/variation: NEKO (Keap1-/-::Nrf2Flox/Flox::K5-Cre)
gender: male
age: 8 weeks
tissue: kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from kidneys using Sepazol-RNA I Super G RNA extraction kit (Nakalai).
|
| Label |
Cy3
|
| Label protocol |
RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
|
| Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner.
|
| Data processing |
The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
|
| |
|
| Submission date |
Jul 25, 2016 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Eri H Kobayashi |
| Organization name |
Tohoku University Graduate School of Medicine
|
| Department |
Medical Biochemistry
|
| Street address |
2-1 Seiryo-machi, Aobaku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE84780 |
Hyperactivation of Nrf2 in early tubular development induces nephrogenic diabetes insipidus |
|
