|
| Status |
Public on Aug 10, 2016 |
| Title |
Huh1_siLSD1_2 |
| Sample type |
RNA |
| |
|
| Source name |
Huh1_siLSD1_1% O2 for 24hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh1
cell type: Hepatocellular Carcinoma Cells
transfected with: siLSD1
|
| Treatment protocol |
Using RNAiMAX (Invitrogen) reagents, Huh-1 cells were reverse-transfected with control siRNA or siLSD1. After 48hrs, cells were incubated under 1% O2 for 24hrs.
|
| Growth protocol |
Huh-1 cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Gibco BRL, Grand Island, NY). Media was supplemented with heat-inactivated 10% fetal bovine serum (GIBCO BRL) and 1× Antibiotic-Antimycotic (GIBCO BRL). Cells were cultured at 37℃ in a humidified incubator under 5% CO2. Hypoxic culture conditions were constructed with O2/CO2 incubator (1%O2, 5% CO2 and 92%-94% N2, MCO-5M, Sanyo, Japan)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was perforemd using QIAGEN RNeasy mini kit according to manufacturer's instruction. Total RNA integrity was determined with ExperionTM RNA StdSens (BIO-RAD).
|
| Label |
Biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification kit (Applied Biosystem/Ambion, Austin, TX, USA) was used to generate biotin-UTP labeled cRNA according to manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Labeled cRNA (750ng) was hybridized to HumanHT-12 v4 chips at 58℃ for 18 hours.
|
| Scan protocol |
After hybridizing procedures, chips were scanned using a BeadArray Reader and BeadScan software (Illumina).
|
| Description |
Gene expression analysis of Huh1 cell line under transfected with siLSD1. At 48hrs after the control siRNA trasfection to Huh-1 cells, the cells were incubated in 1% O2 for 24 hrs. Biological replication #1, Technical replicatipon #2
|
| Data processing |
Data extraction and normalization were elevated by BeadStudio software (v. 3.1.1.0, Illumina); content Descriptor; HumanHT-12_V4_0_R2_15002873_B.bgx
|
| |
|
| Submission date |
Aug 09, 2016 |
| Last update date |
Aug 10, 2016 |
| Contact name |
Yang Suk-Jin |
| E-mail(s) |
sukjini@kribb.re.kr
|
| Organization name |
KRIBB (Korea Research Institute of Bioscience and Biotechnology)
|
| Street address |
125 Gwahak-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE85345 |
Gene expression profiling in LSD1 depleted Huh-1 cell line under hypoxic condition. |
|
