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Sample GSM227526 Query DataSets for GSM227526
Status Public on Oct 05, 2007
Title Stroma normal breast (Nstr101904)
Sample type RNA
 
Source name stroma
Organism Homo sapiens
Characteristics normal
Treatment protocol RNA extraction, cRNA sythesis and Hyb performed according to J. Clin. Invest. 110(2002), pp. 633-641 and Cancer Res. 64(2004), pp. 64-71
Growth protocol RNA extraction, cRNA sythesis and Hyb performed according to J. Clin. Invest. 110(2002), pp. 633-641 and Cancer Res. 64(2004), pp. 64-71
Extracted molecule total RNA
Extraction protocol RNA extraction, cRNA sythesis and Hyb performed according to J. Clin. Invest. 110(2002), pp. 633-641 and Cancer Res. 64(2004), pp. 64-71
Label biotin
Label protocol Biotynylated cRNA were prepared according to the standard affymetrix protocol from 6ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16hr at 45c on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the affymetrix fludics station 400
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanncer G2500A
Description expression of breast tissue
Data processing dCHIP with default settings
 
Submission date Sep 06, 2007
Last update date Aug 28, 2018
Contact name Andrea Richardson
E-mail(s) arichardson@partners.org
Phone 617-732-4764
Organization name Brigham and Women's Hospital
Department Pathology
Street address 75 Francis Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL570
Series (1)
GSE8977 Bone-marrow-derived mesenchymal stem cells promote breast cancer metastasis
Relations
Reanalyzed by GSE20125
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE dchip normalized expression value
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 254.009 P
AFFX-BioB-M_at 243.983 P
AFFX-BioB-3_at 244.507 P
AFFX-BioC-5_at 609.302 P
AFFX-BioC-3_at 627.157 P
AFFX-BioDn-5_at 1264.984 P
AFFX-BioDn-3_at 2162.562 P
AFFX-CreX-5_at 5330.614 P
AFFX-CreX-3_at 5280.175 P
AFFX-DapX-5_at 45.85 A
AFFX-DapX-M_at 30.388 A
AFFX-DapX-3_at -0.824 A
AFFX-LysX-5_at 19.115 A
AFFX-LysX-M_at 22.598 A
AFFX-LysX-3_at 44.81 A
AFFX-PheX-5_at 18.999 A
AFFX-PheX-M_at 220.984 A
AFFX-PheX-3_at 61.219 A
AFFX-ThrX-5_at 47.309 A
AFFX-ThrX-M_at 159.844 A

Total number of rows: 54675

Table truncated, full table size 1073 Kbytes.




Supplementary file Size Download File type/resource
GSM227526.CEL.gz 8.5 Mb (ftp)(http) CEL
GSM227526.dcp.gz 4.1 Mb (ftp)(http) DCP
Processed data included within Sample table
Processed data provided as supplementary file

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