|
Status |
Public on May 01, 2017 |
Title |
Untreated inhalation control 4 days + 24h UT2 |
Sample type |
RNA |
|
|
Source name |
UT2
|
Organism |
Mus musculus |
Characteristics |
exposure: Untreated exposure method: inhalation time: 4d + 24h
|
Treatment protocol |
mice lung tissue samples collected 24h after final exposure. Tissue samples stored in RNAlater in -80ºC
|
Growth protocol |
Female C57BL/6 mice (7-8 weeks old) were housed in groups of four in stainless steel cages, bedded with aspen chip and were provided with standard mouse chow diet and tab water ad lib. 12 h dark/light cycle was used in the animal room temperature being 20-21 degree celcius and humidity of 40-45 %.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolation from the lung samples was done with TRIsure (phenol-chloroform extraction) and the quantity and purity was determined by NanoDrop spectrophotometer. Further quality check of the total RNA was performed with the Agilent BioAnalyzer, according to the manufacturer's recommendations.
|
Label |
Cy5
|
Label protocol |
Samples were amplified using T7 RNA polymerase method and the cRNAs were further labeled with Cy3 and Cy5 dye, according to the manufacturer's recommendations.
|
|
|
Hybridization protocol |
Labeled cRNAs were hybridized to Agilent 2-color 60-mer oligo arrays, , according to the manufacturer's recommendations.
|
Scan protocol |
Slides were washed and scanned with Agilent Microarray scanner G2505C, according to the manufacturer's recommendations.
|
Data processing |
Raw intensity values were obtained from Agilent Feature Extraction software and quality checked according to Agilent standard procedures. The median foreground intensities were imported into R software and analyzed with BioConductor package Limma.
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|
|
Submission date |
Aug 16, 2016 |
Last update date |
May 01, 2017 |
Contact name |
Dario Greco |
E-mail(s) |
dario.greco@tuni.fi
|
Organization name |
Tampere University
|
Department |
Faculty of Medicine and Health Technology
|
Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
|
Street address |
Arvo ylpön Katu 34
|
City |
Tampere |
ZIP/Postal code |
33520 |
Country |
Finland |
|
|
Platform ID |
GPL13912 |
Series (1) |
GSE85711 |
Inhalation and oropharyngeal aspiration exposure to rod-like carbon nanotubes induces highly similar airway inflammation and biological pathways in mouse lungs |
|