|
| Status |
Public on Dec 31, 2017 |
| Title |
Jak1 KO_Sample 6 |
| Sample type |
SRA |
| |
|
| Source name |
LT-HSC
|
| Organism |
Mus musculus |
| Characteristics |
cre status: pos
strain: C57BL/6
tissue: Bone marrow
|
| Treatment protocol |
poly(I:C) injections; to induce gene deletion, 4 doses, 4-6 wks prior to BM harvest
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
Standard poly-A v2 TruSeq RNA protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
ZL7.LTHSC.7
|
| Data processing |
Alignment was performed using the STAR aligner (version 2.3.0e) and mouse genome mm10 as reference.
Aligned results were annotated using the Refseq gene model and featureCounts program from the Subread package for gene level read count.
Genome_build: mm10
Supplementary_files_format_and_content: text file for gene read count
|
| |
|
| Submission date |
Aug 17, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher E Mason |
| E-mail(s) |
chm2042@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Physiology and Biophysics
|
| Lab |
Christopher Mason Lab
|
| Street address |
1305 York Ave
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE85745 |
RNA-seq analysis of Jak1 KO and wt LT-HSCs |
|
| Relations |
| BioSample |
SAMN05583801 |
| SRA |
SRX2033507 |
