As per title for 16 hours, all in the presence of cyclohexamide
Growth protocol
LNCaP cells were grown in charcoal stripped medium
Extracted molecule
total RNA
Extraction protocol
RNAeasy Kit
Label
biotin
Label protocol
According to standard Affymetrix protocol.
Hybridization protocol
According to standard Affymetrix protocol.
Scan protocol
According to standard Affymetrix protocol.
Description
No R1881, 0.1 µM TSA
Data processing
Data was analyzed using GeneSpring. Gene with intensity >250 either unstimulated or stimulated with R1881 was included for analysis. Data was normaled by medium of the chip. No per gene normalization was performed.