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Sample GSM228807 Query DataSets for GSM228807
Status Public on Sep 12, 2007
Title HEL Mlo 1
Sample type RNA
Source name HEL-Ig BM cells
Organism Mus musculus
Characteristics Strain C57BL/6
Treatment protocol HEL treated samples were washed after 5 days, replaced with new media with no IL-7, but 1ug/mL HEL. Control samples were treated the same except no HEL in the media. HEL Mlo samples were sorted for IgMa low, IgDneg. HEL 48h samples were sorted for B220+ IgMahi, GFP+ cells after 48h in HEL. BM 48h Ctrl samples were sorted for B220+ IgMahi GFPneg after 48h in culture with no HEL.
Growth protocol 5 day culture in RPMI + 10% FBS, Pen/Strep, L-glut, 2-ME, 16ng/mL IL-7
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy protocol
Label biotin
Label protocol Ambion cDNA synthesis with T7 oligo dT primer (Array Script). Labeling with biotin-11-CTP and biotin-16-UTP.
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description LET_101042
Data processing MAS 4
Submission date Sep 11, 2007
Last update date Aug 14, 2011
Contact name Lina Tze
Phone 011-61-2-6125-2031
Organization name John Curtin School of Medical Research
Street address
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
Platform ID GPL32
Series (1)
GSE2227 The role of basal immunoglobulin signaling in immature B cell development

Data table header descriptions
VALUE MAS 4 signal

Data table
AFFX-MurIL10_at 3.08
AFFX-MurIL4_at 14.22
AFFX-MurFAS_at 8.975
AFFX-BioB-5_at 256.4
AFFX-BioB-M_at 545.9
AFFX-BioC-5_at 346.3
AFFX-BioDn-5_at 299.1
AFFX-CreX-5_at 3187
AFFX-CreX-3_at 4656
AFFX-ThrX-5_at 2.334
AFFX-BioB-5_st 24.17
AFFX-BioC-3_st 3.252
AFFX-CreX-5_st 21.38
AFFX-CreX-3_st 43.82
AFFX-b-ActinMur/M12481_5_at 5232
AFFX-b-ActinMur/M12481_M_at 5621
AFFX-GapdhMur/M32599_5_at 5085
AFFX-GapdhMur/M32599_M_at 5105
AFFX-GapdhMur/M32599_3_at 4224
AFFX-TransRecMur/X57349_5_at 139.1

Total number of rows: 12654

Table truncated, full table size 194 Kbytes.

Supplementary file Size Download File type/resource
GSM228807.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM228807.CHP.gz 4.2 Mb (ftp)(http) CHP
GSM228807.EXP.gz 465 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

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