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Sample GSM228811 Query DataSets for GSM228811
Status Public on Sep 12, 2007
Title HEL 48h 2
Sample type RNA
 
Source name Rag2-GFP, HEL-Ig BM cells
Organism Mus musculus
Characteristics Strain C57BL/10
Treatment protocol HEL treated samples were washed after 5 days, replaced with new media with no IL-7, but 1ug/mL HEL. Control samples were treated the same except no HEL in the media. HEL Mlo samples were sorted for IgMa low, IgDneg. HEL 48h samples were sorted for B220+ IgMahi, GFP+ cells after 48h in HEL. BM 48h Ctrl samples were sorted for B220+ IgMahi GFPneg after 48h in culture with no HEL.
Growth protocol 5 day culture in RPMI + 10% FBS, Pen/Strep, L-glut, 2-ME, 16ng/mL IL-7
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy protocol
Label biotin
Label protocol Ambion cDNA synthesis with T7 oligo dT primer (Array Script). Labeling with biotin-11-CTP and biotin-16-UTP.
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description LET_1111514
Data processing MAS5.0
 
Submission date Sep 11, 2007
Last update date Aug 14, 2011
Contact name Lina Tze
E-mail(s) Lina.Tze@anu.edu.au
Phone 011-61-2-6125-2031
Organization name John Curtin School of Medical Research
Street address
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
 
Platform ID GPL32
Series (1)
GSE2227 The role of basal immunoglobulin signaling in immature B cell development

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal

Data table
ID_REF VALUE
100000_at 28.27
100001_at 22.06
100002_at 200.78
100003_at 238.02
100004_at 1557.92
100005_at 3044.71
100006_at 200.63
100007_at 3688.17
100008_at 290.22
100009_r_at 17.55
100010_at 868.85
100011_at 2033.49
100012_at 27535.71
100013_at 2997.96
100014_at 900.63
100015_at 97.98
100016_at 767.11
100017_at 442.76
100018_at 984.6
100019_at 102.61

Total number of rows: 12654

Table truncated, full table size 209 Kbytes.




Supplementary file Size Download File type/resource
GSM228811.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM228811.CHP.gz 4.7 Mb (ftp)(http) CHP
GSM228811.EXP.gz 467 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

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