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Sample GSM2292919 Query DataSets for GSM2292919
Status Public on Aug 12, 2017
Title Early differentiating myocytes from healthy donor 7
Sample type RNA
 
Channel 1
Source name Early differentiating myocytes, healthy donor
Organism Homo sapiens
Characteristics disease state: healthy control
individual: Healthy subject 7
gender: male
age: 50
body composition: non-obese
tissue: vastus lateralis
cell type: Early differentiating myocytes
Treatment protocol Cells were not treated
Growth protocol Cells were grown in 20% FBS, 1% PS and 1% FZ in F10/HAM, in 1% matrigel coated culture dishes. At 100% confluent cells, cells were transferred to intermediate medium (DMEM containing 1 g/L glucose, 10% FBS and 1% PS). After 2 days, medium was changed to differentiation media (DMEM containing 4.5 g/L glucose, 2% horse serum and 1% PS) in order to induce differentiation into myotubes (myocytes)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Hy3
Label protocol 500 ng total RNA from sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer
 
Channel 2
Source name Common reference pool (healthy subjects only)
Organism Homo sapiens
Characteristics cell type: isolated satellite cells
Treatment protocol Cells were not treated
Growth protocol Cells were grown in 20% FBS, 1% PS and 1% FZ in F10/HAM, in 1% matrigel coated culture dishes. At 100% confluent cells, cells were transferred to intermediate medium (DMEM containing 1 g/L glucose, 10% FBS and 1% PS). After 2 days, medium was changed to differentiation media (DMEM containing 4.5 g/L glucose, 2% horse serum and 1% PS) in order to induce differentiation into myotubes (myocytes)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Hy5
Label protocol 500 ng total RNA from sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer
 
 
Hybridization protocol The hybridization was performed according to the miRCURY LNA™ microRNA Array instruction manual using a Tecan HS 4800™ hybridization station (Tecan, Austria)
Scan protocol Slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the analyzed using the ImaGene 9.0 software (BioDiscovery, Inc., USA)
Description Healthy subject 7 of 8
C-7-2
Raw files: 0_Exiqon_*.txt represents Hy3 signals, 1_Exiqon_*.txt represents Hy5 signals.
Data processing LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
 
Submission date Aug 25, 2016
Last update date Aug 12, 2017
Contact name Søren Nielsen
E-mail(s) soren_nielsen@inflammation-metabolism.dk
Organization name Rigshospitalet
Department Section 7641
Lab CFAS/CIM
Street address Blegdamsvej 9
City Copenhagen
State/province -
ZIP/Postal code 2100
Country Denmark
 
Platform ID GPL22371
Series (1)
GSE86069 Dysregulation of a miR-23b/27b-p53 axis impairs muscle differentiation in humans with type 2 diabetes

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Hy5/Hy3)

Data table
ID_REF VALUE
148495 2.11
148413 2.32
148038 1.97
146064 1.33
46210 1.58
11040 0.95
46345 1.33
148206 1.26
17858 1.28
147751 1.43
46634 1.09
148504 0.86
10947 1.51
148125 0.80
148371
147631 0.79
42696
145852 1.27
148622 0.61
145865 0.57

Total number of rows: 376

Table truncated, full table size 3 Kbytes.




Supplementary file Size Download File type/resource
GSM2292919_0_Exiqon_14257746_S01.txt.gz 1011.9 Kb (ftp)(http) TXT
GSM2292919_1_Exiqon_14257746_S01.txt.gz 1.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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